City remain on course for an unprecedented quadruple of trophies.
Guardiola expressed his happiness after his team’s 2-0 win against Borussia Monchengladbach in the first leg of the UEFA Champions League round of 16.
Guardiola's men hold a five-point lead at the top of the table with a game in hand over second-placed Manchester United.
Should City win on Sunday they would move 10 points clear of Liverpool having also played one game fewer.
De Bruyne, 29, looked to have sustained a leg muscle injury and was replaced on the hour mark at the Etihad Stadium.
Guardiola's delight at their 2-0 win over Aston Villa on Wednesday was tempered by injuries to playmaker Kevin De Bruyne and defender Kyle Walker.
The 20-year-old midfielder scored his eighth goal of the season as City beat Premier League strugglers Brighton 1-0.
Five Manchester City first team players have tested positive for COVID-19 and will not be involved when they play Chelsea in the Premier League this weekend.
Barcelona star Lionel Messi revealed on Saturday that Pep Guardiola and Luis Enrique played an important role in his career with Barcelona.
Guardiola has challenged his players to work collectively in a bid to rediscover their fluid style of play.
Guardiola said his players could not revel in their 5-0 victory over Burnley on Saturday as hectic match scheduling had sucked some of the joy out of the sport.
Mahrez scored a hat-trick as Manchester City hammered Burnley 5-0 at the Etihad Stadium in the Premier League on Saturday.
The Spanish manager extended his contract with Manchester City, which led to many speculations about the transfer of Barcelona star to the Citizens.
The Spanish manager will be looking forward to win Manchester City’s first ever Champions League trophy during his remaining time with the Citizens.
Guardiola’s side got off to a slugglish start in the Premier League, sitting in 13th place with eight points from five games.
Aguero has been ruled out of Tuesday’s Champions League trip to Olympique Marseille through a hamstring injury and could be out of action for two to four weeks.
Guardiola said striker Sergio Aguero will not hesitate to apologise to a lineswoman if she is unhappy about the Argentine putting his hand on her shoulder.
Manchester City will be in a position to sign Lionel Messi if the opportunity presents itself next season, the club’s chief operating officer said.
The Argentine was linked with a move to Manchester City after he handed a request to Barcelona that he wants to end his contract with the club.
Messi's fellow Argentines back his decision and they are excited by growing reports of a reunion with coach Pep Guardiola at Manchester City.